The technique operates on the principle of . When a molecule absorbs UV or visible light, its electrons move from a low-energy ground state to a higher-energy excited state. The Beer-Lambert Law
(Molar Absorptivity): A constant specific to the substance and wavelength. (Path Length): Typically 1 cm, using a standard cuvette. Essential Components UV-VIS Spectroscopy and Its Applications
This law is the quantitative cornerstone of UV-Vis, relating absorbance ( ) directly to the concentration of the sample ( ) and the path length of the light ( A=ϵ⋅c⋅lcap A equals epsilon center dot c center dot l The technique operates on the principle of