). It is widely used in labs because it is "ratiometric," meaning it provides highly accurate readings by comparing light emitted at two different wavelengths. How it Works
: Observing how new medications affect cell signaling and heart muscle contractions.
Guidelines for the use of flow cytometry and cell sorting ... - PMC Te-AИ™ Fura
: Researchers shine light at two different wavelengths (typically : Light increases when calcium is present. : Light decreases when calcium is present. The Ratio : By calculating the
: Always keep your Fura-2 samples in the dark (wrapped in foil) as much as possible, as the dye will "bleach" and stop working if exposed to too much light. Guidelines for the use of flow cytometry and cell sorting
Fura-2AM is a that allows scientists to see and measure changes in intracellular calcium concentrations (
: The "AM" stands for acetoxymethyl ester . This chemical modification makes the dye membrane-permeant, allowing it to easily slip through the fatty outer layer of a cell. The Ratio : By calculating the : Always
: Many researchers add this detergent to help the dye dissolve better in the cell-loading liquid.