This paper highlights a novel method that combines the ligand-binding domain (LBD) of RAR with the trafficking machinery of the GR, creating a chimeric receptor that moves from the cytoplasm to the nucleus upon ligand binding. 2. Methodology: Design of the GR-RAR Chimera
The chimera is constructed by fusing the ligand-binding domain of the retinoic acid receptor (RAR) to a reporter sequence that allows tracking (e.g., green fluorescent protein). Hem#265 rar
The chimeric receptor is designed to remain in the cytoplasm of untreated cells. This paper highlights a novel method that combines