: Produce the gRNA and Cas protein (often as a Ribonucleoprotein or RNP complex) [15, 31].
According to the Integrated DNA Technologies (IDT) workflow , genome editing typically follows these four major steps [35]: 1. Experimental Design CRISPR: Methods and Protocols
: Directly injecting reagents into embryos or cells [15, 31]. : Produce the gRNA and Cas protein (often
Detailed protocols are documented in academic series like "Methods in Molecular Biology." Key volumes include: CRISPR: Methods and Protocols
: Introduce the CRISPR components into cells using various strategies: Lipofection : Using lipid-based carriers [31].
: Screen for successful mutations using methods like Sanger sequencing or Next-Generation Sequencing (NGS) [12, 31].