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Explain cloning a target sequence into the BbsI sites of pSPgRNA. 47108.rar
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Expression of the chimeric guide RNA in mammalian cells. 47108.rar
Utilizing Addgene Plasmid #47108 (pSPgRNA) for Gene Editing.
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The constant region of the guide RNA that binds to Cas9. Ampicillin Resistance: For selection in bacteria. How to Use This Information